Methylene Blue Bio

T-01

mg/kg dose visualizer

Published per-kilogram figures from the literature scaled to your entered weight, species labeled on every row.

Shows every dose actually used in the human studies this site cites, by indication, converted between total mg and mg/kg for a user-entered body weight, and plots them against the FDA label's toxicity bands and the rodent U-shape. It answers 'what have humans actually been given, in studies, and where does harm start' without prescribing anything.

The U-shaped curve is rat data. The human bands are label toxicity data. Nothing on this chart is a human dosing recommendation; no human cognitive dose-response has ever been mapped.

Weight unit

Published figures scaled to 70 kg

Acquired methemoglobinemia (FDA-approved use)human70 mg (1 mg/kg, IV)

1 mg/kg over 5-30 min; label maximum 2 doses; renal impairment single dose. Hospital emergency, monitored.

Vasoplegic syndrome after cardiac surgery (off-label, randomized study)human105 mg (1.5 mg/kg, IV)

1.5 mg/kg single dose. ICU, monitored.

Cognition imaging trial, healthy adults (single dose)human4 mg/kg (fixed 280 mg, oral)

280 mg once (about 4 mg/kg at 70 kg), USP grade. Research, single dose only.

Fear-extinction augmentation (claustrophobia; PTSD)human3.7 mg/kg (fixed 260 mg, oral)

260 mg immediately after therapy sessions. Research, post-session dosing.

Bipolar disorder adjunct (crossover trials)human2.8 mg/kg/day (fixed 195 mg/day, oral)

195 mg/day (2017) and 300 mg/day vs 15 mg/day (1986). Research, psychiatric supervision.

Alzheimer's phase 2 (different program, same molecule)human2 mg/kg/day (fixed 138 mg/day, oral)

69, 138, or 228 mg/day MTC (methylthioninium chloride); best signal at 138 mg/day; exploratory only. Research.

FDA label harm band, at or above 3 mg/kg single IVhuman210 mg (3 mg/kg, IV)

Hypotension, wheezing, reduced oxygenation reported.

FDA label harm band, at or above 7 mg/kg cumulativehuman490 mg (7 mg/kg, IV)

Paradoxical methemoglobinemia (up to 7%), chest pain, tremor, confusion, staining; effects 2-12 h.

FDA label harm band, at or above 20 mg/kg singlehuman1400 mg (20 mg/kg, IV)

Severe intravascular hemolysis, hyperbilirubinemia, death.

Rat dose-response, 1 mg/kgrat70 mg (1 mg/kg, intraperitoneal)

Improved memory retention; increased brain cytochrome c oxidation.

Rat dose-response, 4 mg/kgrat280 mg (4 mg/kg, intraperitoneal)

Improved habituation and object recognition; extinction retention (5-day dosing).

Rat dose-response, 10 mg/kgrat700 mg (10 mg/kg, intraperitoneal)

No behavioral difference from saline on locomotion or feeding.

Rat dose-response, 50 mg/kgrat3500 mg (50 mg/kg, intraperitoneal)

Reduced running-wheel behavior.

Rat dose-response, 100 mg/kgrat7000 mg (100 mg/kg, intraperitoneal)

Reduced running-wheel behavior.

Scaled mass = published mg/kg x your entered weight. Fixed-dose study rows run the same arithmetic in reverse: mg/kg = the published total dose / your weight. Scaling arithmetic does not transfer evidence between species; dose selection for humans is a clinician's call, not a calculation.

Worked example

Example: at 70 kg, the approved methemoglobinemia dose (1 mg/kg IV) is 70 mg, given once, maximum twice, in a hospital. The single-dose cognition trial gave 280 mg oral, which is 4.0 mg/kg at that same weight, one time, under research supervision. The label documents adverse effects from single doses of 3 mg/kg (210 mg at 70 kg). The gap between 'studied once' and 'harmful' is narrow; that is the point of the chart.

Step by step

  1. Enter body weight and unit.
  2. Read each study row converted to your weight.
  3. Compare study doses against the label hazard bands.
  4. Open a row's citation to read the study before drawing conclusions.

Doses used in human studies, one line each

#IndicationRouteDose as studiedSaltSettingSource
D1Acquired methemoglobinemia (FDA-approved use)IV1 mg/kg over 5-30 min; label maximum 2 doses; renal impairment single dosemethylene blue (ProvayBlue)Hospital emergency, monitoredsource
D2Vasoplegic syndrome after cardiac surgery (off-label, randomized study)IV1.5 mg/kg single dosemethylene blueICU, monitoredsource
D3Septic shock adjunct (off-label, RCT)IVProtocolized IV dosing started within 24 h of shock onsetmethylene blueICU, monitoredsource
D4Cognition imaging trial, healthy adults (single dose)oral280 mg once (about 4 mg/kg at 70 kg), USP grademethylene blue USPResearch, single dose onlysource
D5Fear-extinction augmentation (claustrophobia; PTSD)oral260 mg immediately after therapy sessionsmethylene blueResearch, post-session dosingsource
D6Bipolar disorder adjunct (crossover trials)oral195 mg/day (2017) and 300 mg/day vs 15 mg/day (1986)methylene blueResearch, psychiatric supervisionsource
D7Alzheimer's phase 2 (different program, same molecule)oral69, 138, or 228 mg/day MTC; best signal at 138 mg/day; exploratory onlymethylthioninium chloride (MTC)Researchsource
D8Dementia phase 3s (MODIFIED SALTS, not methylene blue mg)oralLMTM 150-250 mg/day (negative primaries); HMTM 8-16 mg/day (LUCIDITY, primary not demonstrated at 52 wk)LMTM / HMTM (NOT interchangeable with methylene blue)Researchsource

Frequently asked questions

Why does this tool not recommend a dose?

Because none exists to recommend. No trial has established a human dose for any wellness use; the tool shows what studies used and where the label documents harm.

Why are the dementia doses grayed out?

Those trials used chemically modified salts (LMTM, HMTM). Their milligrams are not methylene blue milligrams and cannot be converted.

Is the U-shaped curve human data?

No. The curve is rat data and is labeled rat. The human data on the chart are the label's toxicity bands.

Why is there no reconstitution calculator on this site?

Reconstitution math is for injectable research vials. Methylene blue's wellness form is oral and its approved form is a pre-mixed hospital IV solution, so a reconstitution tool would be the wrong tool for this compound. The suite instead screens interactions, shows studied doses, and checks material grade.

Can these tools tell me a safe dose?

No. No human wellness dose has ever been established, and the label documents harm starting at single doses of 3 mg/kg. The visualizer shows studied doses and hazard bands; the decision belongs with a clinician.

Tools: educational calculators and references only.

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